IL-4 ELISA Kit

Produktnummer ABIN411299

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human IL-4. Es wurde in 22 Publikationen zitiert.

Kurzübersicht für IL-4 ELISA Kit (ABIN411299)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Bindungsspezifität: AA 25-153

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails IL-4 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Human IL-4/Interleukin-4 ELISA Kit to quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: H25-S153

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1.5 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5.8%, 5.8%, 4.2%
Inter-Assay Precision (CV%): 6.2%, 6.1%, 5.5%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-4 ELISA Kit (ABIN411299)

Dang, Xie, Zhou: "Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes." in: Chinese journal of integrative medicine, Vol. 24, Issue 1, pp. 24-31, (2018) (PubMed).

Liu, Piao, Guo, Wang, Sun, Gao, Zheng, Fang: "A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylate on Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 7323129, (2017) (PubMed).

Zang, Jiang, Li, DU, Niu: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).

Jin, Wu, Wang, Peng: "Capsaicin induces immunogenic cell death in human osteosarcoma cells." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 765-770, (2016) (PubMed).

Li, Hu, Wang, Wu, Zhang: "Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 64, Issue 5, pp. 363-71, (2015) (PubMed).

Huang, Wang, Li, Ge, Chen, Chen: "Trichostatin A reduces cisplatin-induced ototoxicity through the STAT6 signaling pathway." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 493-500, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Qiu, Zhu, Lu, Bai, Liu, Zhao, Sun, Zhang, Li, Liu: "The selective mGluR5 agonist CHPG attenuates SO2-induced oxidative stress and inflammation through TSG-6/NF-?B pathway in BV2 microglial cells." in: Neurochemistry international, Vol. 85-86, pp. 46-52, (2015) (PubMed).

Berni Canani, Paparo, Nocerino, Cosenza, Pezzella, Di Costanzo, Capasso, Del Monaco, DArgenio, Greco, Salvatore: "Differences in DNA methylation profile of Th1 and Th2 cytokine genes are associated with tolerance acquisition in children with IgE-mediated cow's milk allergy." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 38, (2015) (PubMed).

Jin, Cheng, Liu, Wu, Wang, Wei, Zhou, Qin, Jia, Zhen et al.: "Improvement of functional recovery by chronic metformin treatment is associated with enhanced alternative activation of microglia/macrophages and increased angiogenesis and neurogenesis following ..." in: Brain, behavior, and immunity, Vol. 40, pp. 131-42, (2014) (PubMed).

Ou, Shi, Xu, Tao: "Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 113, Issue 6, pp. 658-665.e1, (2014) (PubMed).

Huang, Cao: "The protective effects of Shen-Fu injection on experimental acute pancreatitis in a rat model." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 248786, (2014) (PubMed).

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, (2014) (PubMed).

Ozkan, Deveci, Kumbak, Simsek, Ilhan, Sekercioglu, Sapmaz: "What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?" in: Journal of reproductive immunology, Vol. 103, pp. 53-8, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Wang, Feng, Shi, Hao, Yang, Zhang, Kong: "Silencing of miR155 promotes the production of inflammatory mediators in Guillain-Barré syndrome in vitro." in: Inflammation, Vol. 36, Issue 2, pp. 337-45, (2013) (PubMed).

Li, Yu, Ying, Yu: "Antiviral effects of modified dingchuan decoction against respiratory syncytial virus infection in vitro and in an immunosuppressive mouse model." in: Journal of ethnopharmacology, Vol. 147, Issue 1, pp. 238-44, (2013) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Ozkara, Keles, Ilhan, Gungor, Kaygusuz, Alpay: "The relationship between Th1/Th2 balance and 1?,25-dihydroxyvitamin D? in patients with nasal polyposis." in: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, Vol. 269, Issue 12, pp. 2519-24, (2012) (PubMed).

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL4

Hintergrund:

Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Gene Full Name: interleukin 4

Gen-ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation