Interferon gamma ELISA Kit
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- Target Alle Interferon gamma (IFNG) ELISA Kits anzeigen
- Interferon gamma (IFNG)
- Bindungsspezifität
- AA 24-166
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 15.6-1000 pg/mL
- Untere Nachweisgrenze
- 15.6 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IFN gamma
- Marke
- PicoKine™
- Proben
- Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
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Expression system for standard: E.coli
Immunogen sequence: Q24-Q166 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- <2pg/mL
- Benötigtes Material
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
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Expression system for standard: E.coli
Immunogen sequence: Q24-Q166 - Top Product
- Discover our top product IFNG ELISA Kit
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- Applikationshinweise
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Kommentare
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Sequence similarities: Belongs to the type II (or gamma) interferon family.
Tissue Specificity: Released primarily from activated T lymphocytes.
- Plattentyp
- Pre-coated
- Protokoll
- human IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, Q24-Q166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IFN gamma amount of sample captured in plate.
- Testdurchführung
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IFN gamma standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IFN gamma standard solution and each sample be measured in duplicate.
- Testpräzision
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- Sample 1: n=16, Mean(pg/ml): 98.5, Standard deviation: 5.32, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 264, Standard deviation: 9.24, CV(%): 3.5
- Sample 3: n=16, Mean(pg/ml): 579, Standard deviation: 22.00, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 107, Standard deviation: 8.667, CV(%): 8.1
- Sample 2: n=24, Mean(pg/ml): 315, Standard deviation: 20.79, CV(%): 6.6
- Sample 3: n=24, Mean(pg/ml): 589, Standard deviation: 31.81, CV(%): 5.4
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Avoid multiple freeze-thaw cycles.
- Lagerung
- -20 °C,4 °C
- Informationen zur Lagerung
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Haltbarkeit
- 12 months
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MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma." in: Oncotarget, Vol. 7, Issue 35, pp. 57099-57116, (2018) (PubMed).
: "Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mice with Intracranial Gliomas." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3593-3602, (2018) (PubMed).
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: "γδTCR immunoglobulin constant region domain exchange in human αβTCRs improves TCR pairing without altering TCR gene-modified T cell function." in: Molecular medicine reports, Vol. 15, Issue 4, pp. 1555-1564, (2017) (PubMed).
: "Plasma Gelsolin Promotes Proliferation of Mesangial Cell in IgA Nephropathy." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 40, Issue 6, pp. 1473-1486, (2016) (PubMed).
: "A novel anti-GD2/4-1BB chimeric antigen receptor triggers neuroblastoma cell killing." in: Oncotarget, Vol. 6, Issue 28, pp. 24884-94, (2016) (PubMed).
: "Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis." in: Scientific reports, Vol. 5, pp. 18398, (2016) (PubMed).
: "Epigenetic suppression of the antitumor cytotoxicity of NK cells by histone deacetylase inhibitor valproic acid." in: American journal of cancer research, Vol. 6, Issue 3, pp. 600-14, (2016) (PubMed).
: "Cytokine Pattern is Affected by Training Intensity in Women Futsal Players." in: Immune network, Vol. 16, Issue 2, pp. 109-15, (2016) (PubMed).
: "Capsaicin induces immunogenic cell death in human osteosarcoma cells." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 765-770, (2016) (PubMed).
: "Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion." in: Experimental and therapeutic medicine, Vol. 12, Issue 4, pp. 2161-2168, (2016) (PubMed).
: "Evaluation of the inflammatory response in macrophages stimulated with exosomes secreted by Mycobacterium avium-infected macrophages." in: BioMed research international, Vol. 2015, pp. 658421, (2015) (PubMed).
: "Effects of Probiotics on Biomarkers of Oxidative Stress and Inflammatory Factors in Petrochemical Workers: A Randomized, Double-blind, Placebo-controlled Trial." in: International journal of preventive medicine, Vol. 6, pp. 82, (2015) (PubMed).
: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).
: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).
: "Adenovirus-mediated delivery of interferon-γ gene inhibits the growth of nasopharyngeal carcinoma." in: Journal of translational medicine, Vol. 10, pp. 256, (2013) (PubMed).
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MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma." in: Oncotarget, Vol. 7, Issue 35, pp. 57099-57116, (2018) (PubMed).
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- Target Alle Interferon gamma (IFNG) ELISA Kits anzeigen
- Interferon gamma (IFNG)
- Andere Bezeichnung
- IFNG (IFNG Produkte)
- Hintergrund
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Protein Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Synonyms: Interferon gamma,IFN-gamma,Immune interferon,IFNG,
Full Gene Name: Interferon gamma
Cellular Localisation: Secreted. - Gen-ID
- 3458
- UniProt
- P01579
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie
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