BMP2 ELISA Kit

Produktnummer ABIN411262

Maus BMP2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus BMP2.

Kurzübersicht für BMP2 ELISA Kit (ABIN411262)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Bindungsspezifität: AA 283-396

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Osseous Tissue, Cell Culture Supernatant, Serum

Produktdetails BMP2 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-2

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Homologie: Bovine,Canine,Horse,Monkey,Rabbit

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse BMP-2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 314, Standard deviation: 11.3, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 1002, Standard deviation: 39.1, CV(%): 3.9
• Sample 3: n=16, Mean(pg/ml): 2263, Standard deviation: 88.3, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 334, Standard deviation: 24, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 1059, Standard deviation: 74.1, CV(%): 7
• Sample 3: n=24, Mean(pg/ml): 2360, Standard deviation: 177, CV(%): 7.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Andere Bezeichnung: BMP2

Hintergrund:

Protein Function: Induces cartilage and bone formation.

Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Synonyms: Bone morphogenetic protein 2,BMP-2,Bone morphogenetic protein 2A,BMP-2A,Bmp2,Bmp-2,

Full Gene Name: Bone morphogenetic protein 2

Cellular Localisation: Secreted.

Gen-ID: 12156

UniProt: P21274

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation