APOA1 ELISA Kit

Produktnummer ABIN3044715

Human APOA1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human APOA1 und es wurde in 2+ Publikationen zitiert.

Kurzübersicht für APOA1 ELISA Kit (ABIN3044715)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Bindungsspezifität: AA 25-267

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Urine, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails APOA1 ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Verwendungszweck: Sandwich Human APOA1 / Apoa I / Apolipoprotein AI ELISA Kit to quantitate Human APOA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: D25-Q267

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 50 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.6%, 7.3%, 5.2%
Inter-Assay Precision (CV%): 6.2%, 7.9%, 6.1%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für APOA1 ELISA Kit (ABIN3044715)

Ding, Luo, Yu, Zhou, Wu: "Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment." in: Molecular vision, Vol. 24, pp. 252-260, (2018) (PubMed).

Zhao, Li, Zhang, Gao, Pei, Wang, Guo, Yu, Zheng, Wang: "Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma." in: International journal of molecular sciences, Vol. 16, Issue 6, pp. 12669-85, (2016) (PubMed).

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: APOA1

Hintergrund:

Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Gene Full Name: apolipoprotein A1

Gen-ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism