Adiponectin (ADIPOQ) ELISA Kit

Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Synonyms: 30 kDa adipocyte complement-related protein ,Adiponectin, C1Q and collagen domain containing ,Protein Adipoq ,Adipoq ,Acdc ,rCG_36676 ,

Full Gene Name: adiponectin, C1Q and collagen domain containing

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Adiponectin standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(ng/ml): 0.6, Standard deviation: 0.027, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.112, CV(%): 3.2
• Sample 3: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.261, CV(%): 3.9,
• Sample 1: n=24, Mean(ng/ml): 1.2, Standard deviation: 0.114, CV(%): 9.5
• Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.277, CV(%): 7.1
• Sample 3: n=24, Mean(ng/ml): 5.7, Standard deviation: 0.49, CV(%): 8.6