Adiponectin (ADIPOQ) ELISA Kit

Details zu Produkt Nr. ABIN2859334, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACDC
  • ACRP30
  • ADIPQTL1
  • ADPN
  • APM-1
  • APM1
  • GBP28
  • apM-1
  • 30kDa
  • APN
  • Acdc
  • Acrp30
  • Ad
  • adipo
  • apM1
  • ADN
  • acrpl
  • adipo-b
  • adipoql
  • zgc:153584
  • adipoq
  • adiponectin, C1Q and collagen domain containing
  • adiponectin, C1Q and collagen domain containing, b
  • adiponectin, C1Q and collagen domain containing L homeolog
  • ADIPOQ
  • Adipoq
  • adipoqb
  • adipoq.L
Epitop
AA 19-244
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
58
43
33
11
10
8
8
7
6
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
156-10.000 pg/mL
Untere Nachweisgrenze
156 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Adiponectin
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: E.coli
Immunogen sequence: I19-N244
Spezifität Expression system for standard: E.coli
Immunogen sequence: I19-N244
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Antigen
Andere Bezeichnung ADIPOQ (ADIPOQ ELISA Kit Abstract)
Hintergrund

Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Synonyms: 30 kDa adipocyte complement-related protein ,Adiponectin, C1Q and collagen domain containing ,Protein Adipoq ,Adipoq ,Acdc ,rCG_36676 ,

Full Gene Name: adiponectin, C1Q and collagen domain containing

Gen-ID 246253
UniProt Q8K3R4
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plattentyp Pre-coated
Protokoll rat Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(E.coli, I19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Adiponectin amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Adiponectin standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(ng/ml): 0.6, Standard deviation: 0.027, CV(%): 4.5
  • Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.112, CV(%): 3.2
  • Sample 3: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.261, CV(%): 3.9,
  • Sample 1: n=24, Mean(ng/ml): 1.2, Standard deviation: 0.114, CV(%): 9.5
  • Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.277, CV(%): 7.1
  • Sample 3: n=24, Mean(ng/ml): 5.7, Standard deviation: 0.49, CV(%): 8.6
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Adiponectin (ADIPOQ) ELISA Kit (ABIN2859334) Rat Adiponectin PicoKine ELISA Kit standard curve
Produkt verwendet in: El Tabaa, Sokkar, Ramadan, Abd El Salam, Zaid: "Neuroprotective role of Ginkgo biloba against cognitive deficits associated with Bisphenol A exposure: An animal model study." in: Neurochemistry international, Vol. 108, pp. 199-212, 2017 (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, 2015 (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, 2015 (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, 2014 (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, 2014 (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, 2014 (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, 2012 (PubMed).

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