Kallikrein 13 ELISA Kit (Kallikrein-Related Peptidase 13)

Details for Product KLK13 ELISA Kit No. ABIN2859316, Anbieter: Anmelden zum Anzeigen
Antigen
  • KLK-L4
  • KLKL4
  • Egfbp-2
  • mGk-13
  • kallikrein related peptidase 13
  • kallikrein related-peptidase 13
  • KLK13
  • Klk13
Epitop
AA 17-277
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
8
5
Methodentyp
Sandwich ELISA
Detektionsbereich
156-10.000 pg/mL
Untere Nachweisgrenze
156 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK13
Marke PicoKine™
Proben Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: G17-Q277
Spezifität NSO, G17-Q277
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmide, Primers & weitere Kallikrein 13 products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung KLK13 (KLK13 ELISA Kit Abstract)
Hintergrund

Background: Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Synonyms: Kallikrein-13,3.4.21.-,Kallikrein-like protein 4,KLK-L4,KLK13,KLKL4,

Full Gene Name: Kallikrein-13

Cellular Localisation: Secreted.
Gen-ID 26085
UniProt Q9UKR3
Pathways Komplementsystem
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Expressed in prostate, breast, testis and salivary gland.

Plattentyp Pre-coated
Protokoll human KLK13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK13 has been precoated onto 96-well plates. Standards (NSO,G17-Q277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK13 amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK13 standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 1661, Standard deviation: 83.05, CV(%): 5.0
  • Sample 2: n=16, Mean(pg/ml): 3572, Standard deviation: 196.46, CV(%): 5.5
  • Sample 3: n=16, Mean(pg/ml): 6985, Standard deviation: 405.13, CV(%): 5.8,
  • Sample 1: n=24, Mean(pg/ml): 1369, Standard deviation: 71.18, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 4425, Standard deviation: 269.92, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 6594, Standard deviation: 422.01, CV(%): 6.4
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Kallikrein-Related Peptidase 13 (KLK13) ELISA Kit (ABIN2859316) Human KLK13 PicoKine ELISA Kit standard curve