Kallikrein 11 ELISA Kit

Produktnummer ABIN2859314

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Kallikrein 11.

Kurzübersicht für Kallikrein 11 ELISA Kit (ABIN2859314)

Target: Kallikrein 11 (KLK11)

Bindungsspezifität: AA 19-250

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Kallikrein 11 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK11

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: NSO, E19-N250

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E19-N250

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). .

Plattentyp: Pre-coated

Protokoll: human KLK11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been precoated onto 96-well plates. Standards (NSO,E19-N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK11 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK11 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1399, Standard deviation: 68.55, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 3520, Standard deviation: 204.16, CV(%): 5.8
• Sample 3: n=16, Mean(pg/ml): 6635, Standard deviation: 364.92, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 1575, Standard deviation: 92.92, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 4236, Standard deviation: 279.57, CV(%): 6.6
• Sample 3: n=24, Mean(pg/ml): 6751, Standard deviation: 398.30, CV(%): 5.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Kallikrein 11

Target: Kallikrein 11 (KLK11)

Andere Bezeichnung: KLK11

Hintergrund:

Protein Function: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. .

Background: Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Synonyms: Kallikrein-11,hK11,3.4.21.-,Hippostasin,Serine protease 20,Trypsin-like protease,Kallikrein-11 inactive chain 1,Kallikrein-11 inactive chain 2,KLK11,PRSS20, TLSP,UNQ649/PRO1279,

Full Gene Name: Kallikrein-11

Cellular Localisation: Isoform 1: Secreted.

Gen-ID: 11012

UniProt: Q9UBX7

Pathways: Komplementsystem