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NGFR ELISA Kit

NGFR Reaktivität: Human AA 29-250 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Produktnummer ABIN2859309
  • Target Alle NGFR ELISA Kits anzeigen
    NGFR (Nerve Growth Factor Receptor (NGFR))
    Bindungsspezifität
    AA 29-250
    Reaktivität
    • 6
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF16/NGFR
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytische Methode
    Quantitative
    Spezifität
    Sf21, K29-N250
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: sf21
    Immunogen sequence: K29-N250
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    human TNFRSF16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21, K29 - N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 81, Standard deviation: 4.21, CV(%): 5.2
    • Sample 2: n=16, Mean(pg/ml): 330, Standard deviation: 21.45, CV(%): 6.5
    • Sample 3: n=16, Mean(pg/ml): 1560, Standard deviation: 90.48, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 85, Standard deviation: 5.27, CV(%): 6.2
    • Sample 2: n=24, Mean(pg/ml): 360, Standard deviation: 27, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 1350, Standard deviation: 90.45, CV(%): 6.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Zakharyan, Atshemyan, Gevorgyan, Boyajyan: "Nerve growth factor and its receptor in schizophrenia." in: BBA clinical, Vol. 1, pp. 24-9, (2015) (PubMed).

  • Target Alle NGFR ELISA Kits anzeigen
    NGFR (Nerve Growth Factor Receptor (NGFR))
    Andere Bezeichnung
    NGFR (NGFR Produkte)
    Synonyme
    ngfr ELISA Kit, wu:fb38c02 ELISA Kit, wu:fc10h01 ELISA Kit, CD271 ELISA Kit, Gp80-LNGFR ELISA Kit, TNFRSF16 ELISA Kit, p75(NTR) ELISA Kit, p75NTR ELISA Kit, LNGFR ELISA Kit, RNNGFRR ELISA Kit, Tnfrsf16 ELISA Kit, p75 ELISA Kit, NRH1b ELISA Kit, fullback ELISA Kit, nrh1 ELISA Kit, p75ntr ELISA Kit, tnfrsf16 ELISA Kit, p75NGFR ELISA Kit, nerve growth factor receptor ELISA Kit, neurotrophin receptor associated death domain ELISA Kit, nerve growth factor receptor S homeolog ELISA Kit, nerve growth factor receptor (TNFR superfamily, member 16) ELISA Kit, NGFR ELISA Kit, nradd ELISA Kit, Ngfr ELISA Kit, ngfr.S ELISA Kit
    Hintergrund

    Protein Function: Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. .

    Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.

    Synonyms: Tumor necrosis factor receptor superfamily member 16,Gp80-LNGFR,Low affinity neurotrophin receptor p75NTR,Low-affinity nerve growth factor receptor,NGF receptor,p75 ICD,CD271,NGFR,TNFRSF16,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 16

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gen-ID
    4804
    UniProt
    P08138
    Pathways
    NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding
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