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Aggrecan ELISA Kit

Dieses Human Aggrecan ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Aggrecan in Cell Culture Supernatant, Serum und Urine zu quantifizieren.
Produktnummer ABIN2859306

Kurzübersicht für Aggrecan ELISA Kit (ABIN2859306)

Target

Alle Aggrecan (ACAN) ELISA Kits anzeigen
Aggrecan (ACAN)

Bindungsspezifität

AA 20-675

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

1.56-100 ng/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Urine
  • Untere Nachweisgrenze

    1.56 ng/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Aggrecan

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    NSO, V20-G675

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <50pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: V20-G675
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Tissue Specificity: Restricted to cartilages. .

    Plattentyp

    Pre-coated

    Protokoll

    human Aggrecan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been precoated onto 96-well plates. Standards (NSO, V20-G675) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Aggrecan amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Aggrecan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Aggrecan standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(ng/ml): 15, Standard deviation: 0.825, CV(%): 5.5
    • Sample 2: n=16, Mean(ng/ml): 42, Standard deviation: 1.806, CV(%): 4.3
    • Sample 3: n=16, Mean(ng/ml): 74, Standard deviation: 4.588, CV(%): 6.2,
    • Sample 1: n=24, Mean(ng/ml): 16, Standard deviation: 0.944, CV(%): 5.9
    • Sample 2: n=24, Mean(ng/ml): 41, Standard deviation: 2.255, CV(%): 5.5
    • Sample 3: n=24, Mean(ng/ml): 72, Standard deviation: 4.824, CV(%): 6.7

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle Aggrecan (ACAN) ELISA Kits anzeigen

    Aggrecan (ACAN)

    Andere Bezeichnung

    ACAN

    Hintergrund

    Protein Function: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

    Background: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

    Synonyms: Aggrecan core protein,Cartilage-specific proteoglycan core protein,CSPCP,Chondroitin sulfate proteoglycan core protein 1,Chondroitin sulfate proteoglycan 1,Aggrecan core protein 2,ACAN,AGC1, CSPG1, MSK16,

    Full Gene Name: Aggrecan core protein

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.

    Gen-ID

    176

    UniProt

    P16112

    Pathways

    Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
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