CD86 ELISA Kit

Produktnummer ABIN2859285

Dieses Maus CD86 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus CD86 zu quantifizieren.

Kurzübersicht für CD86 ELISA Kit (ABIN2859285)

Target: CD86

Bindungsspezifität: AA 26-245

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails CD86 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD86/B7-2

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: NSO, V26-G245

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: V26-G245

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed on activated B-cells.

Plattentyp: Pre-coated

Protokoll: mouse CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD86 has been precoated onto 96-well plates. Standards (NSO, V26-G245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD86 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD86 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD86 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 0.21, Standard deviation: 0.011, CV(%): 5.3
• Sample 2: n=16, Mean(ng/ml): 1.44, Standard deviation: 0.084, CV(%): 5.9
• Sample 3: n=16, Mean(ng/ml): 2.59, Standard deviation: 0.161, CV(%): 6.2,
• Sample 1: n=24, Mean(ng/ml): 0.23, Standard deviation: 0.013, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.095, CV(%): 6.5
• Sample 3: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.150, CV(%): 6.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Hintergrund:

Protein Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Synonyms: T-lymphocyte activation antigen CD86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1,CD86,Cd86,

Full Gene Name: T-lymphocyte activation antigen CD86

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

Gen-ID: 12524

UniProt: P42082

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation