TIMP1 ELISA Kit (TIMP Metallopeptidase Inhibitor 1)

Details for Product TIMP1 ELISA Kit No. ABIN2859278
AA 25-205
Kits mit alternativen Reaktivitäten:
Sandwich ELISA
31.2-2000 pg/mL
Untere Nachweisgrenze
31.2 pg/mL
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TIMP-1
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: C25-R205
Spezifität Expression system for standard: NSO
Immunogen sequence: C25-R205
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Andere Bezeichnung TIMP1 (TIMP1 ELISA Kit Abstract)

Protein Function: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. .

Background: TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. It was found to reside at Xp11.4-p11.1. This is the first growth factor found to be X-linked. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. What’s more, TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover.

Synonyms: Metalloproteinase inhibitor 1,Collagenase inhibitor 16C8 fibroblast,Erythroid-potentiating activity,EPA,TPA-S1,TPA-induced protein,Tissue inhibitor of metalloproteinases 1,TIMP-1,Timp1,Timp, Timp-1,

Full Gene Name: Metalloproteinase inhibitor 1

Cellular Localisation: Secreted.
Gen-ID 21857
UniProt P12032
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Tissue Specificity: Found in fetal and adult tissues. Highest levels are found in bone. Also found in lung, ovary and uterus.

Plattentyp Pre-coated
Protokoll mouse TIMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-1 has been precoated onto 96-well plates. Standards(NSO, C25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TIMP-1 amount of sample captured in plate.

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TIMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TIMP-1 standard solution and each sample be measured in duplicate.

  • Sample 1: n=16, Mean(pg/ml): 371, Standard deviation: 15.95, CV(%): 4.3
  • Sample 2: n=16, Mean(pg/ml): 602, Standard deviation: 29.5, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 1025, Standard deviation: 36.9, CV(%): 3.6,
  • Sample 1: n=24, Mean(pg/ml): 382, Standard deviation: 24.45, CV(%): 6.4
  • Sample 2: n=24, Mean(pg/ml): 619, Standard deviation: 47.04, CV(%): 7.6
  • Sample 3: n=24, Mean(pg/ml): 1270, Standard deviation: 73.66, CV(%): 5.8
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA Kit (ABIN2859278) Mouse TIMP-1 PicoKine ELISA Kit standard curve
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Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, 2012 (PubMed).