TIMP1 ELISA Kit (TIMP Metallopeptidase Inhibitor 1)

Details for Product TIMP1 ELISA Kit No. ABIN411358, Anbieter: Anmelden zum Anzeigen
Antigen
  • TIMP-1
  • TIMP1
  • DKFZp468A0912
  • CLGI
  • EPA
  • EPO
  • HCI
  • TIMP
  • Timp
  • Clgi
  • TIMP metallopeptidase inhibitor 1
  • tissue inhibitor of metalloproteinase 1
  • TIMP1
  • Timp1
Epitop
AA 24-207
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
37
29
28
12
8
8
8
6
4
4
2
2
2
1
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
156-10000 pg/mL
Untere Nachweisgrenze
156 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIMP-1
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: C24-A207
Spezifität Expression system for standard: NSO
Immunogen sequence: C24-A207
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <5pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmide, Primers & weitere TIMP1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung TIMP1 (TIMP1 ELISA Kit Abstract)
Hintergrund

Protein Function: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro, but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. .

Background: The tissue inhibitor of metalloproteinases 1(TIMP1) is also called erythroid-potentiating activity(EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3(IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.

Synonyms: Metalloproteinase inhibitor 1,Erythroid-potentiating activity,EPA,Fibroblast collagenase inhibitor,Collagenase inhibitor,Tissue inhibitor of metalloproteinases 1,TIMP-1,TIMP1,CLGI, TIMP,

Full Gene Name: Metalloproteinase inhibitor 1

Cellular Localisation: Secreted.
Gen-ID 7076
UniProt P01033
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Tissue Specificity: Detected in rheumatoid synovial fluid (at protein level). .

Plattentyp Pre-coated
Protokoll human TIMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-1 has been precoated onto 96-well plates. Standards(NSO, C24-A207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-1 amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIMP-1 standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(ng/ml): 1.21, Standard deviation: 0.057, CV(%): 4.7
  • Sample 2: n=16, Mean(ng/ml): 3.28, Standard deviation: 0.171, CV(%): 5.2
  • Sample 3: n=16, Mean(ng/ml): 7.06, Standard deviation: 0.424, CV(%): 6,
  • Sample 1: n=24, Mean(ng/ml): 1.34, Standard deviation: 0.071, CV(%): 5.3
  • Sample 2: n=24, Mean(ng/ml): 3.54, Standard deviation: 0.248, CV(%): 7
  • Sample 3: n=24, Mean(ng/ml): 6.32, Standard deviation: 0.43, CV(%): 6.8
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA Kit (ABIN411358) Human TIMP-1 PicoKine ELISA Kit standard curve
Produkt verwendet in: Sollazzo, Forte, Polverelli, Romano, Perricone, Rossi, Ottaviani, Luatti, Martinelli, Vianelli, Cavo, Palandri, Catani: "Crucial factors of the inflammatory microenvironment (IL-1β/TNF-α/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study." in: Oncotarget, Vol. 7, Issue 28, pp. 43974-43988, 2018 (PubMed).

Forte, Salvestrini, Corradi, Rossi, Catani, Lemoli, Cavo, Curti: "The tissue inhibitor of metalloproteinases-1 (TIMP-1) promotes survival and migration of acute myeloid leukemia cells through CD63/PI3K/Akt/p21 signaling." in: Oncotarget, Vol. 8, Issue 2, pp. 2261-2274, 2018 (PubMed).

Liu, Zhong, He, Wei, Zheng, Zhang, Bai, Zhong, Zhong: "Effect of low-dose, long-term roxithromycin on airway inflammation and remodeling of stable noncystic fibrosis bronchiectasis." in: Mediators of inflammation, Vol. 2014, pp. 708608, 2015 (PubMed).

Deng, Ling, Liu, Zhao, Lv: "Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 992-998, 2015 (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, 2015 (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, 2015 (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." in: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, 2015 (PubMed).

Cavdar, Ozbal, Celik, Ergur, Guneli, Ural, Camsari, Guner: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, 2014 (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." in: Mediators of inflammation, Vol. 2014, pp. 510619, 2014 (PubMed).

Li, Zhao, Ren, Xie, Ma, Rong: "Increased expression of matrix metalloproteinase-9 associated with gastric ulcer recurrence." in: World journal of gastroenterology, Vol. 19, Issue 28, pp. 4590-5, 2014 (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, 2013 (PubMed).

Hopps, Lo Presti, Montana, Noto, Averna, Caimi: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." in: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, 2013 (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, 2012 (PubMed).

Allgemeine Veröffentlichungen Anderson, Brown: "Polymorphic X-chromosome inactivation of the human TIMP1 gene." in: American journal of human genetics, Vol. 65, Issue 3, pp. 699-708, 1999 (PubMed).

Spurr, Goodfellow, Docherty: "Chromosomal assignment of the gene encoding the human tissue inhibitor of metalloproteinases to Xp11.1-p11.4." in: Annals of human genetics, Vol. 51, Issue Pt 3, pp. 189-94, 1987 (PubMed).

Gasson, Golde, Kaufman, Westbrook, Hewick, Kaufman, Wong, Temple, Leary, Brown: "Molecular characterization and expression of the gene encoding human erythroid-potentiating activity." in: Nature, Vol. 315, Issue 6022, pp. 768-71, 1985 (PubMed).

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