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KIT Ligand ELISA Kit

Dieses Human KIT Ligand ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human KIT Ligand in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) und Plasma (citrate) zu quantifizieren.
Produktnummer ABIN2859276

Kurzübersicht für KIT Ligand ELISA Kit (ABIN2859276)

Target

Alle KIT Ligand (KITLG) ELISA Kits anzeigen
KIT Ligand (KITLG)

Bindungsspezifität

AA 26-190

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2-2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
  • Untere Nachweisgrenze

    31.2 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SCF

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    E.coli, E26-A190

    Kreuzreaktivität (Details)

    No cross reactivity with other proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: E26-A190
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Plattentyp

    Pre-coated

    Protokoll

    human SCF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCF has been precoated onto 96-well plates. Standards (E.coli, E26-A190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SCF amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human SCF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human SCF standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 115, Standard deviation: 5.75, CV(%): 5.0
    • Sample 2: n=16, Mean(pg/ml): 450, Standard deviation: 23.40, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 1558, Standard deviation: 91.92, CV(%): 5.9,
    • Sample 1: n=24, Mean(pg/ml): 120, Standard deviation: 7.32, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 400, Standard deviation: 26.40, CV(%): 6.6
    • Sample 3: n=24, Mean(pg/ml): 1889, Standard deviation: 122.78, CV(%): 6.5

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle KIT Ligand (KITLG) ELISA Kits anzeigen

    KIT Ligand (KITLG)

    Andere Bezeichnung

    KITLG

    Hintergrund

    Protein Function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

    Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

    Synonyms: Kit ligand,Mast cell growth factor,MGF,Stem cell factor,SCF,c-Kit ligand,Soluble KIT ligand,sKITLG,KITLG,MGF, SCF,

    Full Gene Name: Kit ligand

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

    Gen-ID

    4254

    UniProt

    P21583

    Pathways

    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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