Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IGFBP4 ELISA Kit

Dieses Human IGFBP4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IGFBP4 zu quantifizieren.
Produktnummer ABIN2859270

Kurzübersicht für IGFBP4 ELISA Kit (ABIN2859270)

Target

Alle IGFBP4 ELISA Kits anzeigen
IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Bindungsspezifität

AA 22-258

Reaktivität

  • 6
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

500-32000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    500 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP4

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    NSO, D22-E258

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <20pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: D22-E258
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Plattentyp

    Pre-coated

    Protokoll

    human IGFBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP4 has been precoated onto 96-well plates. Standards (NSO, D22-E258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP4 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 32000pg/mL, 16000pg/mL, 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL human IGFBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP4 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 846, Standard deviation: 43.99, CV(%): 5.2
    • Sample 2: n=16, Mean(pg/ml): 3152, Standard deviation: 179.66, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 12519, Standard deviation: 688.54, CV(%): 5.5,
    • Sample 1: n=24, Mean(pg/ml): 755, Standard deviation: 49.83, CV(%): 6.6
    • Sample 2: n=24, Mean(pg/ml): 3646, Standard deviation: 258.86, CV(%): 7.1
    • Sample 3: n=24, Mean(pg/ml): 13698, Standard deviation: 876.67, CV(%): 6.4

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle IGFBP4 ELISA Kits anzeigen

    IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

    Andere Bezeichnung

    IGFBP4

    Hintergrund

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    Background: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

    Synonyms: Insulin-like growth factor-binding protein 4,IBP-4,IGF-binding protein 4,IGFBP-4,IGFBP4,IBP4,

    Full Gene Name: Insulin-like growth factor-binding protein 4

    Cellular Localisation: Secreted.

    Gen-ID

    3487

    UniProt

    P22692

    Pathways

    WNT Signalweg, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
Sie sind hier:
Chat with us!