ERBB3 ELISA Kit

Produktnummer ABIN2859250

Human ERBB3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ERBB3.

Kurzübersicht für ERBB3 ELISA Kit (ABIN2859250)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Bindungsspezifität: AA 20-643

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails ERBB3 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Human ERBB3/Her3 ELISA Kit to quantitate Human ERBB3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: S20-T643

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5%, 6.6%, 5.9%
Inter-Assay Precision (CV%): 6.9%, 6.6%, 6.9%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: ERBB3

Hintergrund:

Background: Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

Gene Full Name: erb-b2 receptor tyrosine kinase 3

Gen-ID: 2065

UniProt: P21860

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung