Epiregulin ELISA Kit

Produktnummer ABIN2859249

Dieses Maus Epiregulin ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus Epiregulin in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) zu quantifizieren.

Kurzübersicht für Epiregulin ELISA Kit (ABIN2859249)

Target: Epiregulin (EREG)

Bindungsspezifität: AA 53-101

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125-8000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Epiregulin ELISA Kit

Untere Nachweisgrenze: 125 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Epiregulin

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: E.coli, V53-L101

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: V53-L101

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse Epiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Epiregulin has been precoated onto 96-well plates. Standards (E.coli, V53-L101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Epiregulin amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse Epiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Epiregulin standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1257, Standard deviation: 82.96, CV(%): 6.6
• Sample 2: n=16, Mean(pg/ml): 3204, Standard deviation: 189.03, CV(%): 5.9
• Sample 3: n=16, Mean(pg/ml): 5394, Standard deviation: 350.61, CV(%): 6.5,
• Sample 1: n=24, Mean(pg/ml): 1020, Standard deviation: 73.44, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 3562, Standard deviation: 23.865, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 4975, Standard deviation: 368.15, CV(%): 7.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Epiregulin

Target: Epiregulin (EREG)

Andere Bezeichnung: EREG

Hintergrund:

Protein Function: Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (By similarity). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357). .

Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Synonyms: Proepiregulin,Epiregulin,EPR,Ereg,

Full Gene Name: Proepiregulin

Cellular Localisation: Epiregulin: Secreted, extracellular space.

Gen-ID: 13874

UniProt: Q61521

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation