IL-34 ELISA Kit

Produktnummer ABIN2859234

Dieses Human IL-34 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-34 zu quantifizieren.

Kurzübersicht für IL-34 ELISA Kit (ABIN2859234)

Target: IL-34 (IL34) (Interleukin 34 (IL34))

Bindungsspezifität: AA 21-242

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails IL-34 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-34

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: N21-P242

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: N21-P242

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. .

Plattentyp: Pre-coated

Protokoll: human IL-34 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-34 has been precoated onto 96-well plates. Standards(E.coli, N21-P242) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-34 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-34 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-34 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-34 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 514, Standard deviation: 23.13, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 1680, Standard deviation: 65.52, CV(%): 3.9
• Sample 3: n=16, Mean(pg/ml): 2641, Standard deviation: 97.72, CV(%): 3.7,
• Sample 1: n=24, Mean(pg/ml): 535, Standard deviation: 33.17, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 1693, Standard deviation: 103.3, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 2709, Standard deviation: 130, CV(%): 4.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu IL-34

Target: IL-34 (IL34) (Interleukin 34 (IL34))

Andere Bezeichnung: IL34

Hintergrund:

Protein Function: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. .

Background: Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.

Synonyms: Interleukin-34,IL-34,IL34,C16orf77,

Full Gene Name: Interleukin-34

Cellular Localisation: Secreted.

Gen-ID: 146433

UniProt: Q6ZMJ4

Pathways: RTK Signalweg