Selectin E/CD62e ELISA Kit

Produktnummer ABIN2748794

Human Selectin E/CD62e ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Selectin E/CD62e.

Kurzübersicht für Selectin E/CD62e ELISA Kit (ABIN2748794)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 39.1 pg/mL - 2500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma

Produktdetails Selectin E/CD62e ELISA Kit

Untere Nachweisgrenze: 39.1 pg/mL

Verwendungszweck: Sandwich ELISA for quantitative detection of Human CD62E concentrations.

Analytische Methode: Quantitative

Sensitivität: 14 pg/mL

Bestandteile:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Benötigtes Material:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Haltbarkeit: 6-12 months

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: CD62E

Hintergrund: Alternative Names: ELAM1, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E

Gen-ID: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling