Selectin E/CD62e ELISA Kit

Produktnummer ABIN411350

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Selectin E/CD62e. Es wurde in 8 Publikationen zitiert.

Kurzübersicht für Selectin E/CD62e ELISA Kit (ABIN411350)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Bindungsspezifität: AA 22-556

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125 pg/mL - 8000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Produktdetails Selectin E/CD62e ELISA Kit

Untere Nachweisgrenze: 125 pg/mL

Verwendungszweck: Sandwich Human sE-Selectin / CD62E ELISA Kit to quantitate Human SELE in cell culture supernatants, serum and plasma (heparin, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: W22-P556

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 4 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.9%, 6%, 5.9%
Inter-Assay Precision (CV%): 8%, 7.1%, 7.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Selectin E/CD62e ELISA Kit (ABIN411350)

Dehghan, Sabri, Javanmard, Ahmadi, Mansourian: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).

Ahluwalia, Misto, Vozzi, Magliaro, Mattei, Marescotti, Avogaro, Iori: "Systemic and vascular inflammation in an in-vitro model of central obesity." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192824, (2018) (PubMed).

Park, Willoughby, Song, Leutholtz, Koh: "Exercise-induced changes in stress hormones and cell adhesion molecules in obese men." in: Journal of inflammation research, Vol. 11, pp. 69-75, (2018) (PubMed).

Majidinia, Rasmi, Khadem Ansari, Seyed-Mohammadzad, Saboory, Shirpoor: "Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X." in: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 3, pp. 561-566, (2016) (PubMed).

Bilgic, Yilmaz, Bozkurt, Celik, Bilgic, Gurel, Kirbas, Bavbek, Akcay: "Relationship of late arteriovenous fistula stenosis with soluble E-selectin and soluble EPCR in chronic hemodialysis patients with arteriovenous fistula." in: Clinical and experimental nephrology, Vol. 19, Issue 1, pp. 133-9, (2015) (PubMed).

Sabri, Dehghan, Yaghini, Nasiri, Mansourian, Khalifehsoltani: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." in: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: SELE

Hintergrund:

Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Gene Full Name: selectin E

Gen-ID: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling