NOV ELISA Kit

Produktnummer ABIN2748448

Das Rhesusaffen NOV ELISA Kit (ABIN2748448) ist ein Colorimetric ELISA Kit zur Detektion von Rhesusaffen NOV.

Kurzübersicht für NOV ELISA Kit (ABIN2748448)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reaktivität: Rhesusaffen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.02 ng/mL - 6 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails NOV ELISA Kit

Untere Nachweisgrenze: 0.02 ng/mL

Verwendungszweck: Rhesus Macaque NOV ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects Rhesus Macaque and Human NOV. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)
• Adhesive Sealing Film

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard or sample dilutions
• Plastic wrap

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 10 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Andere Bezeichnung: NOV

Hintergrund:

Gene Symbols: NOV, EGK_19231

Protein Name (Synonyms): Protein NOV homolog (Protein NOV-like protein)

Gen-ID: 702196

UniProt: F6WCM6

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding