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NOV ELISA Kit

NOV Reaktivität: Human AA 32-357 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889323
  • Target Alle NOV ELISA Kits anzeigen
    NOV (Nephroblastoma Overexpressed (NOV))
    Bindungsspezifität
    AA 32-357
    Reaktivität
    • 4
    • 4
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2-2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NOV/CCN3
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: T32-M357
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <2pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: T32-M357
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the CCN family.

    Tissue Specificity: Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .

    Plattentyp
    Pre-coated
    Protokoll
    human NOV ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NOV has been precoated onto 96-well plates. Standards(NSO, T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOV is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NOV amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NOV standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NOV standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 154, Standard deviation: 8.78, CV(%): 5.7
    • Sample 2: n=16, Mean(pg/ml): 653, Standard deviation: 40.5, CV(%): 6.2
    • Sample 3: n=16, Mean(pg/ml): 1286, Standard deviation: 61.73, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 197, Standard deviation: 12.61, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 728, Standard deviation: 48.78, CV(%): 6.7
    • Sample 3: n=24, Mean(pg/ml): 1473, Standard deviation: 78.1, CV(%): 5.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle NOV ELISA Kits anzeigen
    NOV (Nephroblastoma Overexpressed (NOV))
    Andere Bezeichnung
    NOV (NOV Produkte)
    Synonyme
    CCN3 ELISA Kit, IBP-9 ELISA Kit, IGFBP-9 ELISA Kit, IGFBP9 ELISA Kit, NOVh ELISA Kit, nov-A ELISA Kit, xnov ELISA Kit, NovH ELISA Kit, C130088N23Rik ELISA Kit, nephroblastoma overexpressed ELISA Kit, nephroblastoma overexpressed L homeolog ELISA Kit, nephroblastoma overexpressed gene ELISA Kit, NOV ELISA Kit, nov.L ELISA Kit, Nov ELISA Kit
    Hintergrund

    Protein Function: Immediate-early protein likely to play a role in cell growth regulation. .

    Background: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

    Synonyms: Protein NOV homolog,NovH,CCN family member 3,Insulin-like growth factor-binding protein 9,IBP-9,IGF-binding protein 9,IGFBP-9,Nephroblastoma-overexpressed gene protein homolog,NOV,CCN3, IGFBP9, NOVH,

    Full Gene Name: Protein NOV homolog

    Cellular Localisation: Secreted.
    Gen-ID
    4856
    UniProt
    P48745
    Pathways
    Smooth Muscle Cell Migration, Growth Factor Binding
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