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CNTF Receptor alpha ELISA Kit

CNTFR Reaktivität: Ratte Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN2748027
  • Target Alle CNTF Receptor alpha (CNTFR) ELISA Kits anzeigen
    CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Rat CNTF R alpha ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Rat CNTF R alpha. Other species not yet determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CNTFR ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CNTF Receptor alpha (CNTFR) ELISA Kits anzeigen
    CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))
    Andere Bezeichnung
    CNTF R alpha (CNTFR Produkte)
    Synonyme
    CNTFR-alpha ELISA Kit, CNTFRALPHA ELISA Kit, Cntfralpha ELISA Kit, cb549 ELISA Kit, fj33e02 ELISA Kit, sb:cb549 ELISA Kit, wu:fj33e02 ELISA Kit, si:bz76a6.1 ELISA Kit, si:dz248f2.1 ELISA Kit, si:rp71-76a6.1 ELISA Kit, ciliary neurotrophic factor receptor ELISA Kit, ciliary neurotrophic factor receptor L homeolog ELISA Kit, CNTFR ELISA Kit, cntfr.L ELISA Kit, Cntfr ELISA Kit, cntfr ELISA Kit
    Hintergrund
    Ciliary neurotrophic factor receptor subunit alpha (CNTF receptor subunit alpha) (CNTFR-alpha)
    Gen-ID
    313173
    UniProt
    Q08406
    Pathways
    JAK-STAT Signalweg, Feeding Behaviour
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