ADIPOQ ELISA Kit

Produktnummer ABIN2747931

Das Ratte ADIPOQ ELISA Kit (ABIN2747931) ist ein Colorimetric ELISA Kit zur Detektion von Ratte ADIPOQ. Das Kit wurde in 2 Publikationen zitiert.

Kurzübersicht für ADIPOQ ELISA Kit (ABIN2747931)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.8-200 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails ADIPOQ ELISA Kit

Untere Nachweisgrenze: 0.8 ng/mL

Verwendungszweck: Rat Adiponectin (ACRP30) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Rat Adiponectin (ADIPOQ/ACRP30)

Sensitivität: 0.8 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für ADIPOQ ELISA Kit (ABIN2747931)

Thakare, Surana: "β-Asarone modulate adipokines and attenuates high fat diet-induced metabolic abnormalities in Wistar rats." in: Pharmacological research, Vol. 103, pp. 227-35, (2016) (PubMed).

Shihabudeen, Roy, James, Thirumurugan: "Chenodeoxycholic acid, an endogenous FXR ligand alters adipokines and reverses insulin resistance." in: Molecular and cellular endocrinology, Vol. 414, pp. 19-28, (2015) (PubMed).

Details zu ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Andere Bezeichnung: Adiponectin

Hintergrund: Gene Names: Adipoq Acdc rCG_36676
Protein names: 30 kDa adipocyte complement-related protein (Adiponectin, C1Q and collagen domain containing) (Protein Adipoq)

Gen-ID: 246253

UniProt: Q8K3R4