OLR1 ELISA Kit

Produktnummer ABIN2708583

Maus OLR1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus OLR1.

Kurzübersicht für OLR1 ELISA Kit (ABIN2708583)

Target: OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12.5 pg/mL - 800 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma

Produktdetails OLR1 ELISA Kit

Untere Nachweisgrenze: 12.5 pg/mL

Verwendungszweck: Sandwich ELISA for quantitative detection of Mouse OLR1 concentrations.

Analytische Methode: Quantitative

Sensitivität: 5 pg/mL

Bestandteile:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Benötigtes Material:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Haltbarkeit: 6-12 months

Details zu OLR1

Target: OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))

Andere Bezeichnung: OLR1

Hintergrund: Alternative Names: CLEC8A, LOX1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1, Lectin-type oxidized LDL receptor 1

Gen-ID: 108078

UniProt: Q9EQ09