VEGFB ELISA Kit

Produktnummer ABIN2703565

Das Human VEGFB ELISA Kit (ABIN2703565) ist ein Colorimetric ELISA Kit zur Detektion von Human VEGFB. Das Kit wurde in 1 Publikation zitiert.

Kurzübersicht für VEGFB ELISA Kit (ABIN2703565)

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.4 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails VEGFB ELISA Kit

Untere Nachweisgrenze: 0.4 ng/mL

Verwendungszweck: Human VEGF-B ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human VEGF-B. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für VEGFB ELISA Kit (ABIN2703565)

Liu, Xu, Jiao, Ren, Li: "Vascular endothelial growth factor B coordinates metastasis of non-small cell lung cancer." in: Tumour biology, Vol. 36, Issue 3, pp. 2185-91, (2015) (PubMed).

Details zu VEGFB

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Andere Bezeichnung: VEGF-B

Hintergrund:

Gene Symbols: VEGFB, VRF

Protein Name (Synonyms): Vascular endothelial growth factor B (VEGF-B) (VEGF-related factor) (VRF)

Gen-ID: 7423

UniProt: P49765

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals