RBP4 ELISA Kit

Produktnummer ABIN2703438

Produktdetails RBP4 ELISA Kit

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.1-30 ng/mL

Untere Nachweisgrenze: 0.1 ng/mL

Applikation: ELISA

Verwendungszweck: Human RBP4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human RBP4. This antibody pair does not cross-react with mouse RBP4.

Sensitivität: 0.1 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples10,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für RBP4 ELISA Kit (ABIN2703438)

Ghareeb, Khalil, Hafez, Bajorath, Ahmed, Sarhan, Elwakeel, El-Demellawy: "Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 361847, (2015) (PubMed).

Details zu RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Andere Bezeichnung: RBP-4 (RBP4 Produkte)

Hintergrund: Gene Names: RBP4 PRO2222
Protein names: Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP) [Cleaved into: Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176)]

Gen-ID: 5950

UniProt: P02753

Pathways: Regulatorische RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response