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Kallikrein 6 ELISA Kit (KLK6) ELISA Kit

KLK6 Reaktivität: Human Colorimetric Sandwich ELISA 0.6-150 ng/mL Cell Culture Supernatant, Plasma, Serum
Pubmed (1 reference)
Produktnummer ABIN2703283
$562.10
Zzgl. Versandkosten $45.00
Lieferung in 2 bis 3 Werktagen
  • Target Alle Kallikrein 6 (KLK6) ELISA Kits anzeigen
    Kallikrein 6 (KLK6)
    Reaktivität
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.6-150 ng/mL
    Untere Nachweisgrenze
    0.6 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human Kallikrein 6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects human KLK6. Other species not determined.
    Sensitivität
    0.6 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften KLK6 ELISA Kit
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Ehrenfeld, Manso, Pavicic, Matus, Borquez, Lizama, Sarmiento, Poblete, Bhoola, Naran, Figueroa: "Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." in: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).

  • Target Alle Kallikrein 6 (KLK6) ELISA Kits anzeigen
    Kallikrein 6 (KLK6)
    Andere Bezeichnung
    KLK6 (KLK6 Produkte)
    Synonyme
    Bssp, Klk7, PRSS18, PRSS9, SP59, hK6, Prss9, AI849898, BSP, Klk29, MSP, Prss18, neurosin, kallikrein related peptidase 6, kallikrein related-peptidase 6, kallikrein-6, KLK6, Klk6, LOC100716801
    Hintergrund
    Gene Names: KLK6 PRSS18 PRSS9
    Protein names: Kallikrein-6 (EC 3.4.21.-) (Neurosin) (Protease M) (SP59) (Serine protease 18) (Serine protease 9) (Zyme)
    Gen-ID
    5653
    UniProt
    Q92876
    Pathways
    Komplementsystem, Regulation of G-Protein Coupled Receptor Protein Signaling
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