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FAS ELISA Kit (TNF Receptor Superfamily, Member 6) ELISA Kit

FAS Reaktivität: Ratte Colorimetric Sandwich ELISA 40-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702995
$562.10
Zzgl. Versandkosten $45.00
Lieferung in 2 bis 3 Werktagen
  • Target Alle FAS ELISA Kits anzeigen
    FAS
    Reaktivität
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    40-10000 pg/mL
    Untere Nachweisgrenze
    40 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Rat Fas (TNFRSF6/Apo-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Rat FAS.
    Sensitivität
    40 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften FAS ELISA Kit
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle FAS ELISA Kits anzeigen
    FAS
    Andere Bezeichnung
    Fas (FAS Produkte)
    Synonyme
    ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, FASL, TNFSF6, APT1LG1, CD178, CD95-L, CD95L, Fas-L, Faslg, Tnfsf6, gld, AI196731, APO1, TNFR6, Tnfrsf6, lpr, Fas cell surface death receptor, Fas ligand, Fas ligand (TNF superfamily, member 6), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta, Fas (TNF receptor superfamily member 6), FAS, FASLG, Fasl, YWHAZ, Fas, fas
    Hintergrund
    Gene Names: Fas Apt1 Tnfrsf6
    Protein names: Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)
    Gen-ID
    246097
    UniProt
    Q63199
    Pathways
    p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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