CD226 ELISA Kit

Produktnummer ABIN2702960

Produktdetails CD226 ELISA Kit

Target: CD226

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 5-600 ng/mL

Untere Nachweisgrenze: 5 ng/mL

Applikation: ELISA

Verwendungszweck: Human DNAM-1 (CD226) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human DNAM1. Other species not determined.

Sensitivität: 5 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2-20 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CD226

Target: CD226

Andere Bezeichnung: DNAM-1 (CD226 Produkte)

Synonyme: CD226 ELISA Kit, cd226 ELISA Kit, zgc:158817 ELISA Kit, BC051526 ELISA Kit, DNAM-1 ELISA Kit, DNAM1 ELISA Kit, Pta1 ELISA Kit, TLiSA1 ELISA Kit, PTA1 ELISA Kit, CD226 molecule ELISA Kit, CD226 antigen ELISA Kit, CD226 ELISA Kit, cd226 ELISA Kit, Cd226 ELISA Kit

Hintergrund: Gene Names: CD226 DNAM1
Protein names: CD226 antigen (DNAX accessory molecule 1) (DNAM-1) (CD antigen CD226)

Gen-ID: 10666

UniProt: Q15762

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints