LY9 ELISA Kit
Kurzübersicht für LY9 ELISA Kit (ABIN2702892)
Target
Alle LY9 (CD229) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
-
-
Untere Nachweisgrenze
- 80 pg/mL
-
Verwendungszweck
- Human CD229 (SLAMF3) ELISA Kit for cell culture supernatants, plasma, and serum samples.
-
Analytische Methode
- Quantitative
-
Spezifität
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1 .
-
Sensitivität
- 80 pg/mL
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
-
-
Applikationshinweise
- Recommended Dilution for serum and plasma samples3 fold
-
Probenmenge
- 100 μL
-
Plattentyp
- Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Lagerung
- -20 °C
-
Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
Haltbarkeit
- 6 months
-
-
- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
-
Andere Bezeichnung
- CD229
-
Hintergrund
-
Gene Names: LY9 CDABP0070
Protein names: T-lymphocyte surface antigen Ly-9 (Cell surface molecule Ly-9) (Lymphocyte antigen 9) (SLAM family member 3) (SLAMF3) (Signaling lymphocytic activation molecule 3) (CD antigen CD229)
-
Gen-ID
- 4063
-
UniProt
- Q9HBG7
-
Pathways
- BCR Signaling
Target Alle LY9 (CD229) ELISA Kits anzeigen
-