Apolipoprotein C-II ELISA Kit
-
- Target Alle Apolipoprotein C-II (APOC2) ELISA Kits anzeigen
- Apolipoprotein C-II (APOC2)
-
Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 1.5-100 ng/mL
- Untere Nachweisgrenze
- 1.5 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human ApoC2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
- Sensitivität
- 1.5 ng/mL
- Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Zu unserem meistverkauften APOC2 ELISA Kit
- Top Product
- Discover our top product APOC2 ELISA Kit
-
-
- Applikationshinweise
- Recommended Dilution for serum and plasma samples20,000 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Aufbereitung der Proben
-
For example, add 2 μL of serum/plasma into a tube with 198.0 μL Assay Diluent C to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL Assay Diluent C to prepare a final 20,000 fold diluted sample.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
-
- Target Alle Apolipoprotein C-II (APOC2) ELISA Kits anzeigen
- Apolipoprotein C-II (APOC2)
- Andere Bezeichnung
- ApoC2 (APOC2 Produkte)
- Synonyme
- apoc2 ELISA Kit, apoC-II ELISA Kit, fb98g08 ELISA Kit, wu:fb52e03 ELISA Kit, wu:fb71a04 ELISA Kit, wu:fb98g08 ELISA Kit, APOC2 ELISA Kit, APO-CII ELISA Kit, APOC-II ELISA Kit, RGD1560725 ELISA Kit, APOCII ELISA Kit, Apo-CII ELISA Kit, ApoC-II ELISA Kit, apolipoprotein C-II ELISA Kit, apolipoprotein C2 ELISA Kit, apoc2 ELISA Kit, APOC2 ELISA Kit, Apoc2 ELISA Kit
- Hintergrund
-
Gene Names: APOC2 APC2
Protein names: Apolipoprotein C-II (Apo-CII) (ApoC-II) (Apolipoprotein C2) [Cleaved into: Proapolipoprotein C-II (ProapoC-II)]
- Gen-ID
- 344
- UniProt
- P02655
- Pathways
- Lipid Metabolism
-