Apolipoprotein C-II ELISA Kit

Produktnummer ABIN2702831

Human Apolipoprotein C-II ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Apolipoprotein C-II.

Kurzübersicht für Apolipoprotein C-II ELISA Kit (ABIN2702831)

Target: Apolipoprotein C-II (APOC2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.5-100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails Apolipoprotein C-II ELISA Kit

Untere Nachweisgrenze: 1.5 ng/mL

Verwendungszweck: Human ApoC2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF

Sensitivität: 1.5 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples20,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

For example, add 2 μL of serum/plasma into a tube with 198.0 μL Assay Diluent C to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL Assay Diluent C to prepare a final 20,000 fold diluted sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Apolipoprotein C-II

Target: Apolipoprotein C-II (APOC2)

Andere Bezeichnung: ApoC2

Hintergrund: Gene Names: APOC2 APC2
Protein names: Apolipoprotein C-II (Apo-CII) (ApoC-II) (Apolipoprotein C2) [Cleaved into: Proapolipoprotein C-II (ProapoC-II)]

Gen-ID: 344

UniProt: P02655

Pathways: Lipid Metabolism