CCL2 ELISA Kit

Produktnummer ABIN2690571

Dieses Human CCL2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CCL2 zu quantifizieren. Dieses ELISA Kit wurde in 3+ Publikationen zitiert.

Kurzübersicht für CCL2 ELISA Kit (ABIN2690571)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Supernatant

Produktdetails CCL2 ELISA Kit

Verwendungszweck: The Human MCP-1 ELISA Kit is for the quantitative determination of human MCP-1 in serum, plasma, and cell culture supernatant.

Analytische Methode: Quantitative

Bestandteile: Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

BD OptEIA™ Human MCP-1 ELISA Kit

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

Referenzen für CCL2 ELISA Kit (ABIN2690571)

Rollins: "Chemokines." in: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).

Rollins, Stier, Ernst, Wong: "The human homolog of the JE gene encodes a monocyte secretory protein." in: Molecular and cellular biology, Vol. 9, Issue 11, pp. 4687-95, (1990) (PubMed).

Capsoni, Minonzio, Ongari, Zanussi: "A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes." in: Journal of immunological methods, Vol. 120, Issue 1, pp. 125-31, (1989) (PubMed).

Details zu CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Andere Bezeichnung: MCP-1

Hintergrund: Human monocyte chemoattractant protein-1 (MCP-1), previously known as MCAF (monocyte chemotactic and activating factor), is a member of the CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion