IL-10 ELISA Kit

Produktnummer ABIN2648753

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Schwein IL-10.

Kurzübersicht für IL-10 ELISA Kit (ABIN2648753)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails IL-10 ELISA Kit

Sensitivität: 7 pg/ml

Produktmerkmale: Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-4). All IL-10 family members are secreted α-helical proteins. Porcine IL-10 is a secreted, possibly glycosylated, polypeptide with an 18 kDa molecular weight (5). Based on human studies, porcine IL-10 is likely to circulate as a nondisulfide-linked homodimer (3). Porcine IL-10 is synthesized as a 175 amino acid (aa) precursor with an 18 aa signal sequence and a 157 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which form two intrachain disulfide bridges (6). Mature porcine IL-10 shows 71 % , 70 %, 76 %, 75 %, 77 %, and 71 % aa sequence identity to rat (7), mouse (8), human (9), guinea pig (10), canine (11), and cotton rat (12) IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells (13), cytotoxic CD8+ T cells secreting Th2-like cytokines (14), CD4+CD45RA- (memory) Th1 and Th2 cells (15), macrophages (16), monocytes (17), CD5+ and CD5- B cells (18, 19), dendritic cells (20, 21), hepatic stellate (Ito cells) (22), keratinocytes (23), melanoma cells (24), mast cells (25), placental cytotrophoblasts (26), and fetal erythroblasts (27).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: Swine Interleukin 10 (IL-10) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any IL-10 present is bound by the immobilized antibody and the detection antibody specific for IL-10 is binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: IL-10

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints