IGFBP4 ELISA Kit
Kurzübersicht für IGFBP4 ELISA Kit (ABIN2648687)
Target
Alle IGFBP4 ELISA Kits anzeigenReaktivität
Nachweismethode
Applikation
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Verwendungszweck
- IGFBP-4 ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) in biological fluids.
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Analytische Methode
- Quantitative
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Sensitivität
- 0.625 ng/ml
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Produktmerkmale
- The IGFBP-4 ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Probenmenge
- 50 μL
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Testdauer
- 2 h
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Plattentyp
- Pre-coated
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Protokoll
- The biotinylated antibody binds to the streptavidin coated microtiter plate. The IGFBP-4 of the standards/samples binds to this antibody. The biotinylated antibody and the standards/ samples are incubated on the microtiter plate at the same time. After a washing step the bound IGFBP-4, is then detected by a horseradish peroxidase-conjugated anti-IGFBP-4 antibody. After a second washing step the conjugate is added and incubated. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of IGFBP-4.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
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Andere Bezeichnung
- IGFBP-4
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Pathways
- WNT Signalweg, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
Target Alle IGFBP4 ELISA Kits anzeigen
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