Interferon gamma ELISA Kit

Produktnummer ABIN2648684

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Interferon gamma.

Kurzübersicht für Interferon gamma ELISA Kit (ABIN2648684)

Target: Interferon gamma (IFNG)

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails Interferon gamma ELISA Kit

Verwendungszweck: Human Interferon Gamma (IFN-γ) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interferon Gamma (IFN-γ) concentrations in cell culture supernates, serum, and plasma.

Analytische Methode: Quantitative

Sensitivität: 7 pg/ml

Produktmerkmale: Human Interferon Gamma (IFN-γ) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: Interferon Gamma (IFN-γ) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody- IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFN-gamma

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie