CD86 ELISA Kit

Produktnummer ABIN2648551

Human CD86 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human CD86.

Kurzübersicht für CD86 ELISA Kit (ABIN2648551)

Target: CD86

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails CD86 ELISA Kit

Verwendungszweck: Human CD86 ELISA is to be used for the in-vitro quantitative determination CD86 in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD86.

Analytische Methode: Quantitative

Sensitivität: 0.6 U/ml

Produktmerkmale: Human CD86 ELISA Assay kit is for Research Use Only.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The CD86 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD86 antigen and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD86 present in the samples.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation