KIT ELISA Kit

Produktnummer ABIN2648535

Dieses Human KIT ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human KIT zu quantifizieren.

Kurzübersicht für KIT ELISA Kit (ABIN2648535)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails KIT ELISA Kit

Verwendungszweck: Human CD117 ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (CD117) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant CD117.

Analytische Methode: Quantitative

Sensitivität: 0.095 ng/ml

Produktmerkmale: Human CD117 ELISA Assay kit is for Research Use Only.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 1 - 2 h

Plattentyp: Pre-coated

Protokoll: The CD117 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD117 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD117 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD117 antigen and a biotinylated monoclonal antibody specific for CD117 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD117 present in the samples.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: CD117 / C-KIT

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity