IL-7 ELISA Kit

Produktnummer ABIN2112003

Dieses Human IL-7 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-7 zu quantifizieren.

Kurzübersicht für IL-7 ELISA Kit (ABIN2112003)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.5-500 pg/mL

Applikation: ELISA

Produktdetails IL-7 ELISA Kit

Untere Nachweisgrenze: 1.5 pg/mL

Sensitivität: < 1 pg/mL

Bestandteile: IL-7 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
IL-7 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This IL-7 ELISA is a ready-to-use 3.5-hour solid phase immunoassay readily capable of measuring IL-7 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 2000pg/ml. This assay has shown no cross-reactivity with other cytokines tested, and is expected to be used effectively for further investigations into the relationship between IL-7 and the various conditions mentioned.This IL-7 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-7 and incubated. If present, IL-7 will bind and become immobilized by the antibody pre-coated on the wells and then become -sandwiched- by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL- 7 and other components of the sample. In order to quantitatively determine the amount of IL-7 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl- benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-7, biotin- conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: Interleukin-7

Pathways: JAK-STAT Signalweg