IL-5 ELISA Kit

Produktnummer ABIN2112000

Dieses Maus IL-5 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus IL-5 zu quantifizieren.

Kurzübersicht für IL-5 ELISA Kit (ABIN2112000)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 3.12-200 pg/mL

Applikation: ELISA

Produktdetails IL-5 ELISA Kit

Untere Nachweisgrenze: 3.12 pg/mL

Marke: BioAssay™

Sensitivität: < 0.8 pg/mL

Bestandteile: MOUSE IL-5 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-5 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
TMB SUBSTRATE
STOP SOLUTION

Anwendungsinformationen

Protokoll: Mouse IL-5 is a heamotapoietic cytokine, mainly produced by T-helper 2 cells and mast cells. The cytokine is comprised of two identical 113 amino acid units linked by disulfide bond. The gene for mouse IL-5 is located at chromosome 11 in conjunction with genes for IL4 and other T-helper 2 cytokines such as IL-13, GM-CSF, IL-3. IL-5 regulates B cell proliferation, eosinophil maturation and activation. IL-5 receptor is a heterodimer with a cytokine specific alpha unit, and a signal transduction beta subunit that is identical to the beta subunit of receptors for IL-3 and GM-CSF. IL-5 receptor alpha (IL-5Ra) is expressed by eosinophils, B cells and basophils in member-bound form and soluble form. While the membrane bound form participates in the activation of eosinophils and B cells, the soluble form of IL-5Ra has an antagonist effect to IL-5. Increased IL-5 expression causes chronic IgE response and hypereosinophilic syndromes. Elevated IL-5 level is found in allergic diseases such as allergic rhinitis, asthma and allergic conjunctivitis. This ELISA kit provides a tool for studying IL-5 expression and regulation in animal model. This mouse IL-5 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-5. When standards or samples are added to the appropriate microtiter plate wells, mouse IL-5 in the standards or samples will be immobilized by the precoated antibody during incubation. Then, a biotin-conjugated antibody preparation specific for mouse IL-5 is added to each well and incubated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: Interleukin-5

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour