Interleukin 17a ELISA Kit

Produktnummer ABIN2111994

Dieses Maus Interleukin 17a ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus Interleukin 17a zu quantifizieren.

Kurzübersicht für Interleukin 17a ELISA Kit (ABIN2111994)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails Interleukin 17a ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Sensitivität: < 2 pg/mL

Bestandteile: ANTI-MOUSE IL-17A MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-17 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This mouse IL-17A ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure mouse IL-17A levels in cell culture supernatant and mouse serum in the range of 0 to 1000pg/ml. It showed no cross reactivity with other cytokines tested. This mouse IL-17A ELISA is expected to be effectively used for further investigations into the relationship between IL-17A and the various conditions mentioned. This mouse IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for mouse IL-17A was added and incubated. Mouse IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-17A and other components in the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and aTMB(3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: Interleukin-17A