Interleukin 17a ELISA Kit

Produktnummer ABIN411314

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Interleukin 17a. Es wurde in 18 Publikationen zitiert.

Kurzübersicht für Interleukin 17a ELISA Kit (ABIN411314)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Bindungsspezifität: AA 20-155

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails Interleukin 17a ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich Human IL-17/IL-17A ELISA Kit to quantitate Human IL17A in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: I20-A155

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.2%, 7.1%, 4.5%
Inter-Assay Precision (CV%): 8.7%, 7.2%, 5.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Interleukin 17a ELISA Kit (ABIN411314)

Badal, Kumar, Paul, Dayal, Bhansali, Bhadada, Kumar, Sachdeva et al.: "Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in ..." in: The Indian journal of medical research, Vol. 145, Issue 6, pp. 767-776, (2018) (PubMed).

Min, Yan, Wang, Landeck, Chen, Li, Cai, Zheng, Man: "Rottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies." in: PLoS ONE, Vol. 12, Issue 12, pp. e0190051, (2018) (PubMed).

Yuksel, Kaplan, Ates, Kilic, Kilic, Suna, Ates, Kayacetin: "The role of soluble tumor necrosis factor like weak inducer of apoptosis and interleukin-17A in the etiopathogenesis of celiac disease: A cross-sectional study." in: Medicine, Vol. 95, Issue 26, pp. e3937, (2017) (PubMed).

Choghakhori, Abbasnezhad, Hasanvand, Amani: "Inflammatory cytokines and oxidative stress biomarkers in irritable bowel syndrome: Association with digestive symptoms and quality of life." in: Cytokine, Vol. 93, pp. 34-43, (2017) (PubMed).

Romano, Sollazzo, Trabanelli, Barone, Polverelli, Perricone, Forte, Luatti, Cavo, Vianelli, Jandus, Palandri, Catani: "Mutations in JAK2 and Calreticulin genes are associated with specific alterations of the immune system in myelofibrosis." in: Oncoimmunology, Vol. 6, Issue 10, pp. e1345402, (2017) (PubMed).

Jiang, Chen, Wang, Lin, Hu, Fang: "Changes in interleukin-17 and transforming growth factor beta 1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma." in: Translational pediatrics, Vol. 2, Issue 4, pp. 154-9, (2016) (PubMed).

Cam, Karagoz, Muftuoglu, Bigi, Emirzeoglu, Celik, Ozgun, Tuncel, Top: "The inflammatory cytokine interleukin-23 is elevated in lung cancer, particularly small cell type." in: Contemporary oncology (Poznań, Poland), Vol. 20, Issue 3, pp. 215-9, (2016) (PubMed).

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, (2015) (PubMed).

Lu, Zeng, Han, Lin, Long, Dan, Zhou, Chen: "Overexpression and selectively regulatory roles of IL-23/IL-17 axis in the lesions of oral lichen planus." in: Mediators of inflammation, Vol. 2014, pp. 701094, (2014) (PubMed).

Chai, Yang, Gao, Guo, Li, Fan, Wu, Xue, Cai, Jiang, Qin, Zhang, Ke: "Decreased percentages of regulatory T cells are necessary to activate Th1-Th17-Th22 responses during acute rejection of the peripheral nerve xenotransplantation in mice." in: Transplantation, Vol. 98, Issue 7, pp. 729-37, (2014) (PubMed).

Jin, Wan, Chen, Chen, Zhang, Deng, Zhang, Xiong, Xin: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).

Ozkan, Deveci, Kumbak, Simsek, Ilhan, Sekercioglu, Sapmaz: "What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?" in: Journal of reproductive immunology, Vol. 103, pp. 53-8, (2014) (PubMed).

Ou, Shi, Xu, Tao: "Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 113, Issue 6, pp. 658-665.e1, (2014) (PubMed).

Sereshki, Gharagozloo, Ostadi, Ghahiri, Roghaei, Mehrabian, Andalib, Hassanzadeh, Hosseini, Rezaei: "Variations in T-helper 17 and Regulatory T Cells during The Menstrual Cycle in Peripheral Blood of Women with Recurrent Spontaneous Abortion." in: International journal of fertility & sterility, Vol. 8, Issue 1, pp. 59-66, (2014) (PubMed).

Jiang, Chen, Wang, Lin, Hu, Fang: "[Changes in IL-17 and TGF-?1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma]." in: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, Vol. 15, Issue 8, pp. 604-8, (2013) (PubMed).

Xu, Tao, Zhang, Zhang: "Neutralization of interleukin-17 attenuates high fat diet-induced non-alcoholic fatty liver disease in mice." in: Acta biochimica et biophysica Sinica, Vol. 45, Issue 9, pp. 726-33, (2013) (PubMed).

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, (2013) (PubMed).

Liu, Ge, Ma, Mei, Liu, Zhang, Ren, Liao, Pu, Wang, You: "Interleukin-17 and prostaglandin E2 are involved in formation of an M2 macrophage-dominant microenvironment in lung cancer." in: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, Vol. 7, Issue 7, pp. 1091-100, (2012) (PubMed).

Details zu Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: IL17A

Hintergrund:

Background: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57 % identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

Gene Full Name: interleukin 17A

Gen-ID: 3605

UniProt: Q16552