YAP1 ELISA Kit

Produktnummer ABIN2044861

Maus YAP1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus YAP1.

Kurzübersicht für YAP1 ELISA Kit (ABIN2044861)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails YAP1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.056 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Yes Associated Protein 1 (YAP1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Yes Associated Protein 1 (YAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Yes Associated Protein 1 (YAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Yes Associated Protein 1 (YAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Yes Associated Protein 1 (YAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Andere Bezeichnung: Yes Associated Protein 1 (YAP1)

Pathways: MAPK Signalweg, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha