WIF1 ELISA Kit

Produktnummer ABIN2044847

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human WIF1.

Kurzübersicht für WIF1 ELISA Kit (ABIN2044847)

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails WIF1 ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 6.9 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: WNT Inhibitory Factor 1 (WIF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu WIF1

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Pathways: WNT Signalweg, Positive Regulation of fat Cell Differentiation