UBE2I ELISA Kit

Produktnummer ABIN2044739

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human UBE2I.

Kurzübersicht für UBE2I ELISA Kit (ABIN2044739)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails UBE2I ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.053 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ubiquitin Conjugating Enzyme E2I (UBE2I) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2I (UBE2I), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Conjugating Enzyme E2I (UBE2I) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Andere Bezeichnung: Ubiquitin Conjugating Enzyme E2I (UBE2I)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway