KIT Ligand ELISA Kit

Produktnummer ABIN2044605

Dieses Rind (Kuh) KIT Ligand ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Rind (Kuh) KIT Ligand zu quantifizieren.

Kurzübersicht für KIT Ligand ELISA Kit (ABIN2044605)

Target: KIT Ligand (KITLG)

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails KIT Ligand ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 5.7 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Stem Cell Factor (SCF) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stem Cell Factor (SCF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Stem Cell Factor (SCF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: Stem Cell Factor (SCF)

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung