RIPK3 ELISA Kit

Produktnummer ABIN2044403

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte RIPK3.

Kurzübersicht für RIPK3 ELISA Kit (ABIN2044403)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RIPK3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.055 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Receptor Interacting Serine Threonine Kinase 3 (RIPK3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 3 (RIPK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Receptor Interacting Serine Threonine Kinase 3 (RIPK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Andere Bezeichnung: Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades