PDGFC ELISA Kit

Produktnummer ABIN2044239

Dieses Ratte PDGFC ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte PDGFC zu quantifizieren.

Kurzübersicht für PDGFC ELISA Kit (ABIN2044239)

Target: PDGFC (Platelet-Derived Growth Factor C (PDGFC))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails PDGFC ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.113 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Platelet Derived Growth Factor C (PDGFC) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor C (PDGFC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor C (PDGFC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor C (PDGFC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Platelet Derived Growth Factor C (PDGFC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PDGFC

Target: PDGFC (Platelet-Derived Growth Factor C (PDGFC))

Andere Bezeichnung: Platelet Derived Growth Factor C (PDGFC)

Substanzklasse: Viral Protein

Pathways: RTK Signalweg, Platelet-derived growth Factor Receptor Signaling