Peroxidasin ELISA Kit

Produktnummer ABIN2044201

Human Peroxidasin ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Peroxidasin.

Kurzübersicht für Peroxidasin ELISA Kit (ABIN2044201)

Target: Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails Peroxidasin ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Peroxidasin Homolog (PXDN) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxidasin Homolog (PXDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxidasin Homolog (PXDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxidasin Homolog (PXDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Peroxidasin Homolog (PXDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Peroxidasin

Target: Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))