Periostin ELISA Kit

Produktnummer ABIN2044199

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus Periostin.

Kurzübersicht für Periostin ELISA Kit (ABIN2044199)

Target: Periostin (POSTN)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.62-40 ng/mL

Applikation: ELISA

Produktdetails Periostin ELISA Kit

Untere Nachweisgrenze: 0.62 ng/mL

Sensitivität: < 0.207 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Periostin (POSTN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periostin (POSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Periostin (POSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periostin (POSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Periostin (POSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Periostin

Target: Periostin (POSTN)