NKAP ELISA Kit

Produktnummer ABIN2044093

Ratte NKAP ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte NKAP.

Kurzübersicht für NKAP ELISA Kit (ABIN2044093)

Target: NKAP (NFKB Activating Protein (NKAP))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails NKAP ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: NFKB Activating Protein (NKAP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB Activating Protein (NKAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFKB Activating Protein (NKAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB Activating Protein (NKAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of NFKB Activating Protein (NKAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NKAP

Target: NKAP (NFKB Activating Protein (NKAP))

Pathways: Stem Cell Maintenance, Phosphorylierungen bei SARS-CoV-2 Infektion